StartupBlink

42 Top startups in Frederick for November 2026

We rank RoosterBio, KnowledgeNet.ai and BioFactura in Frederick along with 39 other startups based on an algorithm that scores startups based on total investment, employee count, and quarterly website traffic. Frederick startup ecosystem ranks #471 globally and #126 in United States.

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Best Startups in Frederick for 2026

1st
RoosterBio logo
RoosterBioSB Score: 417

RoosterBio is a biotechnology company, offers synthetic biology and regenerative medicine. The team at RoosterBio leads the field of new-thinkers in stem cell research and commercialization. The company is committed to simplifying, de-risking, and de-bottlenecking your lab, liberating you to do more high-impact research. It was founded in 2012 and headquartered in Frederick, Maryland.

View Full Page
Website
www.roosterbio.com
Employee range
51 – 100
Web Traffic (Monthly)
—
Funding amount
$41M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
2nd
KnowledgeNet.ai logo
KnowledgeNet.aiSB Score: 344

KnowledgeNet.ai is a provider of knowledge intelligence solutions. KnowledgeNet.ai engages in network collaboration that revolutionizes how organizations use intellectual capital.

View Full Page
Website
knowledgenet.ai
Employee range
11 – 50
Web Traffic (Monthly)
—
Funding amount
$9.9M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
3rd
BioFactura logo
BioFacturaSB Score: 324

BioFactura has developed the StableFast™ Biomanufacturing Platform, a novel system to rapidly generate stable NS0 cell lines for biopharmaceutical manufacturing. The elements of this system include a chemically-defined, serum- and cholesterol-free production medium and pre-adapted parental cells, modular expression vectors that utilize a patented selection strategy, and a proprietary growth supplement that permits rapid generation and cloning of cell lines. This platform is simple to use, fast, and productive. A clonal production cell line can be developed and scaled up to yield over one gram of product in less than eight weeks.

View Full Page
Website
www.biofactura.com
Employee range
1 – 10
Web Traffic (Monthly)
—
Funding amount
$76.2M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
4th
EDUsys Global logo
EDUsys GlobalSB Score: 318

EDUsys Global is an education training provider conducting courses which are recognized by globally reputed institutions like Project Management Institute, Pennsylvania, U.S.A.; Human Resources Certification Institute, Alexandria, Virginia, U.S.A.; APMG International, Buckinghamshire, U.K., and more. It is an affiliate of VMEdu. EDUsys Global offers knowledge enrichment, skill sharpening, and competitive edge through online courses and tests that spans a vast spectrum of conventional and emerging domains of learning and work. EDUsys Global’s learning management and back office systems have given it scalability in terms of its ability to offer better courses and cater to various domains of professional education where there is a lack of good-quality content and study methodology. More than 100,000 individuals and over 5000 companies across the world have benefitted from EDUsys Global’s time-tested and consistently proven education delivery models. It is based in Frederick, M.D.

View Full Page
Website
www.edusysglobal.com
Employee range
51 – 100
Web Traffic (Monthly)
—
Funding amount
$13.2M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
5th
Akonni Biosystems logo
Akonni BiosystemsSB Score: 313

Akonni Biosystems is a biotechnology company that develops and manufactures, genetic testing devices. Its devices are designed for complex infectious and human health diseases like Methicillin-resistant Staphylococcus aureus (MRSA), multi-drug-resistant tuberculosis (MDR-TB), and influenza sub-typing with oseltamivir-resistance detection. It was founded in 2003 and headquartered in Maryland, United States.

View Full Page
Website
www.akonni.com
Employee range
11 – 50
Web Traffic (Monthly)
—
Funding amount
$56.8M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
6th
Fugue logo
FugueSB Score: 301

Fugue ensures cloud infrastructure stays in continuous compliance with enterprise security policies. Its solution identifies cloud infrastructure security risks and compliance violations and ensures that they are never repeated. Fugue provides baseline drift detection and automated remediation to eliminate data breaches, and powerful visualization and reporting tools to easily demonstrate compliance. Fugue automates compliance audits with out-of-the-box frameworks for HIPAA, GDPR, CIS Benchmark, and NIST 800-53, and supports custom policies. Fugue works with CI/CD pipelines to ensure compliance and prevent unauthorized change. Customers like Sparkpost, PBS, and SAP NS2 rely on Fugue to protect against cloud risks and enforce compliance.

View Full Page
Website
fugue.co
Employee range
1 – 10
Web Traffic (Monthly)
—
Funding amount
$85M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
7th
Veralox Therapeutics logo

Veralox Therapeutics develops small molecule therapeutics that treat the underlying pathologies of thrombosis and type one diabetes. Based on an understanding of the molecular mechanisms of these diseases, these efforts will lead to new treatment paradigms and better outcomes for patients.

View Full Page
Website
www.veralox.com
Employee range
1 – 10
Web Traffic (Monthly)
—
Funding amount
$46M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
8th
FESCO Energy logo
FESCO EnergySB Score: 274

FESCO Energy simplifies the acquisition, contracting, deployment, and management of energy solutions for government agencies and organizations. They offer customized energy solutions for federal and commercial customers.

View Full Page
Website
www.fescoenergy.com
Employee range
11 – 50
Web Traffic (Monthly)
—
Funding amount
$11.9M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
9th
Cerus Fitness logo
Cerus FitnessSB Score: 266

Our cloud-based AI platform represents the new era of earth intelligence as it sources images and data from satellite, aerial, and underwater sources on-demand, and seamlessly fuses and projects 3D visualizations with AI-driven analytics to facilitate faster and more informed decision-making for any place on earth. We help clients navigate through uncertainty, change and crisis.

View Full Page
Website
cerusfitness.com
Employee range
11 – 50
Web Traffic (Monthly)
—
Funding amount
$1M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
10th
Tellor logo
TellorSB Score: 265

Tellor is a decentralized oracle on the Ethereum network that provides trustless access to high-value off-chain data. By utilizing a network of staked miners who compete to retrieve off-chain pricing data, Tellor’s system is both secure and easy to implement.

View Full Page
Website
www.tellor.io
Employee range
11 – 50
Web Traffic (Monthly)
—
Funding amount
$400K
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
11th
Precision Medical Pte. Ltd. logo

Precision for Medicine is dedicated to supporting the unprecedented acceleration in medical research and the explosion of biomarker and translational sciences. These dynamically changing fields require novel services and an integration of expertise not commonly found in traditional research organizations. We’ve assembled an extraordinary array of talent and services to enable our pharmaceutical and life sciences clients to take advantage of new advancements in science and stay ahead of regulatory changes. Our team encompasses scientists, clinical trial experts and technical specialists that support our trailblazing clients through: *A state-of-the-art biorepository and laboratory *Biomarker identification through analysis of ’omics and clinical data *A comprehensive approach to companion diagnostic product development *Fully integrated and global clinical trial capabilities

View Full Page
Website
www.precisionformedicine.com
Employee range
1,001 – 5,000
Web Traffic (Monthly)
—
Funding amount
—
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—

Total amount:25

Most Funded Startups in Frederick for 2026

The top three most funded startups in Frederick have raised total funding of more than USD 218M. Fugue has 0.12% more funding than BioFactura.
1st
Fugue logo
FugueSB Score: 301

Fugue ensures cloud infrastructure stays in continuous compliance with enterprise security policies. Its solution identifies cloud infrastructure security risks and compliance violations and ensures that they are never repeated. Fugue provides baseline drift detection and automated remediation to eliminate data breaches, and powerful visualization and reporting tools to easily demonstrate compliance. Fugue automates compliance audits with out-of-the-box frameworks for HIPAA, GDPR, CIS Benchmark, and NIST 800-53, and supports custom policies. Fugue works with CI/CD pipelines to ensure compliance and prevent unauthorized change. Customers like Sparkpost, PBS, and SAP NS2 rely on Fugue to protect against cloud risks and enforce compliance.

View Full Page
Website
fugue.co
Employee range
1 – 10
Web Traffic (Monthly)
—
Funding amount
$85M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
2nd
BioFactura logo
BioFacturaSB Score: 324

BioFactura has developed the StableFast™ Biomanufacturing Platform, a novel system to rapidly generate stable NS0 cell lines for biopharmaceutical manufacturing. The elements of this system include a chemically-defined, serum- and cholesterol-free production medium and pre-adapted parental cells, modular expression vectors that utilize a patented selection strategy, and a proprietary growth supplement that permits rapid generation and cloning of cell lines. This platform is simple to use, fast, and productive. A clonal production cell line can be developed and scaled up to yield over one gram of product in less than eight weeks.

View Full Page
Website
www.biofactura.com
Employee range
1 – 10
Web Traffic (Monthly)
—
Funding amount
$76.2M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
3rd
Akonni Biosystems logo
Akonni BiosystemsSB Score: 313

Akonni Biosystems is a biotechnology company that develops and manufactures, genetic testing devices. Its devices are designed for complex infectious and human health diseases like Methicillin-resistant Staphylococcus aureus (MRSA), multi-drug-resistant tuberculosis (MDR-TB), and influenza sub-typing with oseltamivir-resistance detection. It was founded in 2003 and headquartered in Maryland, United States.

View Full Page
Website
www.akonni.com
Employee range
11 – 50
Web Traffic (Monthly)
—
Funding amount
$56.8M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—

Total amount:25

Top Hiring Startups in Frederick by Employee Number

The top three hiring startups in Frederick have an median employee size that is between [51-100]
1st
Precision Medical Pte. Ltd. logo

Precision for Medicine is dedicated to supporting the unprecedented acceleration in medical research and the explosion of biomarker and translational sciences. These dynamically changing fields require novel services and an integration of expertise not commonly found in traditional research organizations. We’ve assembled an extraordinary array of talent and services to enable our pharmaceutical and life sciences clients to take advantage of new advancements in science and stay ahead of regulatory changes. Our team encompasses scientists, clinical trial experts and technical specialists that support our trailblazing clients through: *A state-of-the-art biorepository and laboratory *Biomarker identification through analysis of ’omics and clinical data *A comprehensive approach to companion diagnostic product development *Fully integrated and global clinical trial capabilities

View Full Page
Website
www.precisionformedicine.com
Employee range
1,001 – 5,000
Web Traffic (Monthly)
—
Funding amount
—
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
2nd
RoosterBio logo
RoosterBioSB Score: 417

RoosterBio is a biotechnology company, offers synthetic biology and regenerative medicine. The team at RoosterBio leads the field of new-thinkers in stem cell research and commercialization. The company is committed to simplifying, de-risking, and de-bottlenecking your lab, liberating you to do more high-impact research. It was founded in 2012 and headquartered in Frederick, Maryland.

View Full Page
Website
www.roosterbio.com
Employee range
51 – 100
Web Traffic (Monthly)
—
Funding amount
$41M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
3rd
EDUsys Global logo
EDUsys GlobalSB Score: 318

EDUsys Global is an education training provider conducting courses which are recognized by globally reputed institutions like Project Management Institute, Pennsylvania, U.S.A.; Human Resources Certification Institute, Alexandria, Virginia, U.S.A.; APMG International, Buckinghamshire, U.K., and more. It is an affiliate of VMEdu. EDUsys Global offers knowledge enrichment, skill sharpening, and competitive edge through online courses and tests that spans a vast spectrum of conventional and emerging domains of learning and work. EDUsys Global’s learning management and back office systems have given it scalability in terms of its ability to offer better courses and cater to various domains of professional education where there is a lack of good-quality content and study methodology. More than 100,000 individuals and over 5000 companies across the world have benefitted from EDUsys Global’s time-tested and consistently proven education delivery models. It is based in Frederick, M.D.

View Full Page
Website
www.edusysglobal.com
Employee range
51 – 100
Web Traffic (Monthly)
—
Funding amount
$13.2M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—
4th
KnowledgeNet.ai logo
KnowledgeNet.aiSB Score: 344

KnowledgeNet.ai is a provider of knowledge intelligence solutions. KnowledgeNet.ai engages in network collaboration that revolutionizes how organizations use intellectual capital.

View Full Page
Website
knowledgenet.ai
Employee range
11 – 50
Web Traffic (Monthly)
—
Funding amount
$9.9M
National Rank(United States)
—
Regional Rank(North America)
—
Global Rank(Worldwide)
—

Total amount:42

Explore More Top Startups

Explore the Startup Ecosystem of Frederick

National Rank

(United States)

126th

new

Regional Rank

(North America)

163rd

18

Global Rank

(WorldWide)

471st

44

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2026 National Rank CityGlobal RankTotal Score & National ShareAnnual Growth

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