711 Top startups in Czechia for April 2025

We rank Kiwi, MALL.cz and Knihobot in Czechia along with 708 other startups based on an algorithm that scores startups based on total investment, employee count, and quarterly website traffic. Czechia startup ecosystem ranks #32 globally and #3 in Eastern Europe.

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Top Unicorn Startups in Czechia for 2025

Rohlik is the only unicorn in Czechia raising more than USD 1.2B. It is the undefined ranked unicorn in Eastern Europe.
SB Score:544

Rohlik offers grocery delivery with same-day service in just 90 minutes. Experience convenient and fast shopping from the comfort of your home.

Industry:
Ecommerce & Retail
Subindustry:
Ecommerce

City Rank (Prague)

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Total amount:1

Best Startups in Czechia for 2025

1st

Kiwi
SB Score:659

Kiwi.com is an innovative online travel platform that simplifies trip planning by offering a wide range of flight and accommodation options. Users can easily compare prices, book their travel arrangements, and enjoy a seamless booking experience.

Industry:
Social & Leisure
Subindustry:
Travel

City Rank (Brno)

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SB Score:609

MALL.cz is an online retailer in the Czech Republic. The site also operates in Germany, Poland, Hungary and Slovakia. The company runs more than 45 e-shops in the region with 1,200 product categories.

Industry:
Ecommerce & Retail
Subindustry:
Ecommerce

City Rank (Prague)

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SB Score:542

Choice is a SaaS platform designed for hotels and restaurants, enabling them to digitize their operations, boost revenue, and cut costs. With its user-friendly tools, businesses can streamline processes and enhance guest experiences, driving efficiency and profitability in the hospitality industry.

Industry:
Software & Data
Subindustry:
Software

City Rank (Prague)

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SB Score:515

Apify is the one-stop shop for all your web scraping, data extraction, and robotic process automation needs.

Industry:
Software & Data
Subindustry:
Apps

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SB Score:490

Better Stack revolutionizes software delivery with devops solutions that leverage logs and monitoring as a powerful tool. Accelerate your development process and gain a competitive edge with our innovative technology.

Industry:
Software & Data
Subindustry:
Enterprise Technology

City Rank (Prague)

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SB Score:481

BudgetBakers.com is a growing Prague-based startup, focused on keeping your budget low and helping you learn how to manage your money.

Industry:
Software & Data
Subindustry:
Apps

City Rank (Prague)

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9th

FAVI
SB Score:469

FAVI is an online search engine for furniture and home decor, established in 2015. Operating in the Czech Republic, Slovakia, Poland, Romania, and Hungary, FAVI offers users a vast selection of inspiring products, with easy filtering by style, category, and more to simplify their decorating journey.

Industry:
Ecommerce & Retail
Subindustry:
Ecommerce & Retail-Other

City Rank (Prague)

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SB Score:468

Enterprise blockchain made easy. Tatum is a developer tool making all developers blockchain developers . Integrate the value of blockchain in days. Innovate and scale seamlessly and securely.

Industry:
Fintech
Subindustry:
Blockchain

City Rank (Brno)

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Regional Rank (Eastern Europe)

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11th

SOTIO
SB Score:454

SOTIO Biotech is shaping the future of cancer immunotherapies by translating compelling science into patient benefit.

Industry:
Healthtech
Subindustry:
Biotechnology

City Rank (Prague)

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Regional Rank (Eastern Europe)

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Total amount:712

Top 323 Software & Data Startups in Czechia for 2025

The top three Software & Data startups in Czechia have raised more than USD 131M. Czechia startup ecosystem ranks #28 globally for the Software & Data industry.
SB Score:542

Choice is a SaaS platform designed for hotels and restaurants, enabling them to digitize their operations, boost revenue, and cut costs. With its user-friendly tools, businesses can streamline processes and enhance guest experiences, driving efficiency and profitability in the hospitality industry.

Industry:
Software & Data
Subindustry:
Software

City Rank (Prague)

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Regional Rank (Eastern Europe)

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SB Score:515

Apify is the one-stop shop for all your web scraping, data extraction, and robotic process automation needs.

Industry:
Software & Data
Subindustry:
Apps

City Rank (Prague)

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Regional Rank (Eastern Europe)

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SB Score:490

Better Stack revolutionizes software delivery with devops solutions that leverage logs and monitoring as a powerful tool. Accelerate your development process and gain a competitive edge with our innovative technology.

Industry:
Software & Data
Subindustry:
Enterprise Technology

City Rank (Prague)

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Regional Rank (Eastern Europe)

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Total amount:323

Top 67 Ecommerce & Retail Startups in Czechia for 2025

The top three Ecommerce & Retail startups in Czechia have raised more than USD 205M. Czechia startup ecosystem ranks #25 globally for the Ecommerce & Retail industry.
SB Score:609

MALL.cz is an online retailer in the Czech Republic. The site also operates in Germany, Poland, Hungary and Slovakia. The company runs more than 45 e-shops in the region with 1,200 product categories.

Industry:
Ecommerce & Retail
Subindustry:
Ecommerce

City Rank (Prague)

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Regional Rank (Eastern Europe)

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Total amount:67

Top 32 Healthtech Startups in Czechia for 2025

The top three Healthtech startups in Czechia have raised more than USD 661M. Czechia startup ecosystem ranks #28 globally for the Healthtech industry.
SB Score:454

SOTIO Biotech is shaping the future of cancer immunotherapies by translating compelling science into patient benefit.

Industry:
Healthtech
Subindustry:
Biotechnology

City Rank (Prague)

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SB Score:358

Get personalized nutrition advice and delicious recipes with a virtual nutritionist and chef in your pocket. Receive expert guidance on healthy eating and meal planning to help you reach your wellness goals.

Industry:
Healthtech
Subindustry:
Health Care

City Rank (Prague)

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SB Score:333

Puravia offers a wide range of food supplements, including omega-3 fatty acids, multivitamins, minerals, proteins, and more. Their products also include medicinal mushrooms, cannabinoids, and antioxidants for overall health and wellness. Choose Puravia for a natural way to support your body's needs.

Industry:
Healthtech
Subindustry:
Health Care

City Rank (Prague)

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Total amount:32

Most Funded Startups in Czechia for 2025

The top three most funded startups in Czechia have raised total funding of more than USD 933M. SOTIO has 2.58% more funding than MALL.cz.
SB Score:454

SOTIO Biotech is shaping the future of cancer immunotherapies by translating compelling science into patient benefit.

Industry:
Healthtech
Subindustry:
Biotechnology

City Rank (Prague)

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SB Score:609

MALL.cz is an online retailer in the Czech Republic. The site also operates in Germany, Poland, Hungary and Slovakia. The company runs more than 45 e-shops in the region with 1,200 product categories.

Industry:
Ecommerce & Retail
Subindustry:
Ecommerce

City Rank (Prague)

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SB Score:354

This startup streamlines the heating installation process by digitizing every stage, from lead generation and sales to installation and after-sales support. By integrating advanced technology, it enhances efficiency, improves customer experience, and simplifies management for both homeowners and professionals in the heating industry.

Industry:
Energy & Environment
Subindustry:
Energy

City Rank (Prague)

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Regional Rank (Eastern Europe)

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Total amount:712

Top Hiring Startups in Czechia by Employee Number

The top three hiring startups in Czechia have an median employee size that is between [1001-5000]
SB Score:354

Moravia specializes in translation, localization, and testing services, empowering companies to effectively enter global markets. Their comprehensive globalization solutions ensure that products and services are expertly tailored to meet the linguistic and functional needs of customers worldwide.

Industry:
Software & Data
Subindustry:
Software and Data-Other

City Rank (Brno)

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Regional Rank (Eastern Europe)

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2nd

Kiwi
SB Score:659

Kiwi.com is an innovative online travel platform that simplifies trip planning by offering a wide range of flight and accommodation options. Users can easily compare prices, book their travel arrangements, and enjoy a seamless booking experience.

Industry:
Social & Leisure
Subindustry:
Travel

City Rank (Brno)

0

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Regional Rank (Eastern Europe)

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SB Score:258

We develop software that helps customers manage routine agenda and legislative obligations

Industry:
Software & Data
Subindustry:
Software

City Rank (Brno)

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Regional Rank (Eastern Europe)

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Total amount:711

Explore the Startup Ecosystem of Czechia

Population

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10.5 Million

new

Regional Rank

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3rd

2

Global Rank

(WorldWide)

32nd

3

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Top Cities for Startups in Czechia - Ranked by StartupBlink

StartupBlink ranks the startup ecosystems of more than 100 countries and 1,000 cities. Download our latest Global Startup Ecosystem Report.
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2024 National Rank CityGlobal Rank
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Total Score

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Quantity Score
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Quality Score
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Business Score
1st
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89th
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6
8.622
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190th
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47
2.799
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754th
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146
0.208
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974th
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0.125
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1273rd
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0.051
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