Dropping the price of human genome sequencing to under $1000 will open a new era of personalized medicine. Sequencing requires preparation of uniformly sized DNA libraries and accounts for significant fraction of the final cost.
We are working on developing of novel approach of enzymatic generation of uniformly sized DNA pools. This will be a platform for quick and inexpensive library preparation for NextGen Sequencing.
Without going into technical details, the main principle is control of termination: DNA polymerase will be forced to “freeze” when the product of certain, predefined length is synthesized. One particular implementation of the technology is “size-specific PCR”. Contrary to regular PCR, which is sequence-specific, size-specific PCR will be able to generate uniformly sized pools of DNA for only a fraction of current time, cost and amount of starting material. The product can be made barcoaded during the reaction, thus giving single step library prep method for NGS.